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anti py845 egfr  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti py845 egfr
    Anti Py845 Egfr, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 282 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/egfr+py845/Phospho-EGF+Receptor+(Tyr845)+Antibody/pm39817537-483-10-13
    Average 95 stars, based on 282 article reviews
    anti py845 egfr - by Bioz Stars, 2026-10
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    Related Articles

    Chromatin Immunoprecipitation:

    Article Title: Glioblastoma mutations alter EGFR dimer structure to prevent ligand bias.
    Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Human research participants Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Cell Signaling Technology (CST): CST #2231 - Rabbit polyclonal against EGFR pY845 (used at 1:1000 dilution for Western blotting) CST #3777 - Rabbit monoclonal IgG against EGFR pY1068 (used at 1:1000 dilution for Western blotting) 3 nature portfolio | reporting sum m ary M arch 2021 CST #4407 - Rabbit monoclonal IgG against EGFR pY1173 (used at 1:1000 dilution for Western blotting) CST #9106 - Mouse monoclonal IgG1 against pT202/pY204 of ERK1/2 (used at 1:1000 dilution for Western blotting phospho ERK) CST #4696 - Mouse monoclonal IgG1 against (total) ERK1/2 (used at 1:1000 dilution for Western blotting total ERK) CST #3972 - Rabbit polyclonal against Grb2 (used at 1:1000 dilution for Western blotting) CST #7076 - Horse anti-mouse IgG (heavy and light chain) antibody conjugated to horseradish peroxidase (HRP) - used at 1:10,000 dilution as secondary antibody in Western blotting with mouse primaries. .. R&D AF231 - Goat polyclonal IgG against total EGFR (used at 1:1000 dilution for Western blotting total EGFR) R&D HAF017 - Rabbit anti-goat IgG conjugated to horseradish peroxidase (HRP) - used at 1:1000 dilution as secondary antibody in Western blotting with goat primaries.

    Article Title: Developmental mosaicism underlying EGFR-mutant lung cancer presenting with multiple primary tumors.
    Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used EGFR (Cell Signaling Technologies #4267, multiple lots, 1:500 dilution); EGFR pY845 (Cell Signaling Technologies #6963, multiple lots, 1:500 dilution); Akt1/2 (Cell Signaling Technologies #9272, multiple lots, 1:1000 dilution); Akt pS473 (Cell Signaling Technologies #4060, multiple lots, 1:500 dilution); Vinculin (Sigma Aldrich MAB3574 clone VIIF9, multiple lots, 1:2000 dilution). ..

    Article Title: Developmental mosaicism underlying EGFR -mutant lung cancer presenting with multiple primary tumors
    Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used EGFR (Cell Signaling Technologies #4267, multiple lots, 1:500 dilution); EGFR pY845 (Cell Signaling Technologies #6963, multiple lots, 1:500 dilution); Akt1/2 (Cell Signaling Technologies #9272, multiple lots, 1:1000 dilution); Akt pS473 (Cell Signaling Technologies #4060, multiple lots, 1:500 dilution); Vinculin (Sigma Aldrich MAB3574 clone VIIF9, multiple lots, 1:2000 dilution). ..

    Flow Cytometry:

    Article Title: Glioblastoma mutations alter EGFR dimer structure to prevent ligand bias.
    Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Human research participants Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Cell Signaling Technology (CST): CST #2231 - Rabbit polyclonal against EGFR pY845 (used at 1:1000 dilution for Western blotting) CST #3777 - Rabbit monoclonal IgG against EGFR pY1068 (used at 1:1000 dilution for Western blotting) 3 nature portfolio | reporting sum m ary M arch 2021 CST #4407 - Rabbit monoclonal IgG against EGFR pY1173 (used at 1:1000 dilution for Western blotting) CST #9106 - Mouse monoclonal IgG1 against pT202/pY204 of ERK1/2 (used at 1:1000 dilution for Western blotting phospho ERK) CST #4696 - Mouse monoclonal IgG1 against (total) ERK1/2 (used at 1:1000 dilution for Western blotting total ERK) CST #3972 - Rabbit polyclonal against Grb2 (used at 1:1000 dilution for Western blotting) CST #7076 - Horse anti-mouse IgG (heavy and light chain) antibody conjugated to horseradish peroxidase (HRP) - used at 1:10,000 dilution as secondary antibody in Western blotting with mouse primaries. .. R&D AF231 - Goat polyclonal IgG against total EGFR (used at 1:1000 dilution for Western blotting total EGFR) R&D HAF017 - Rabbit anti-goat IgG conjugated to horseradish peroxidase (HRP) - used at 1:1000 dilution as secondary antibody in Western blotting with goat primaries.

    Article Title: Developmental mosaicism underlying EGFR-mutant lung cancer presenting with multiple primary tumors.
    Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used EGFR (Cell Signaling Technologies #4267, multiple lots, 1:500 dilution); EGFR pY845 (Cell Signaling Technologies #6963, multiple lots, 1:500 dilution); Akt1/2 (Cell Signaling Technologies #9272, multiple lots, 1:1000 dilution); Akt pS473 (Cell Signaling Technologies #4060, multiple lots, 1:500 dilution); Vinculin (Sigma Aldrich MAB3574 clone VIIF9, multiple lots, 1:2000 dilution). ..

    Article Title: Developmental mosaicism underlying EGFR -mutant lung cancer presenting with multiple primary tumors
    Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used EGFR (Cell Signaling Technologies #4267, multiple lots, 1:500 dilution); EGFR pY845 (Cell Signaling Technologies #6963, multiple lots, 1:500 dilution); Akt1/2 (Cell Signaling Technologies #9272, multiple lots, 1:1000 dilution); Akt pS473 (Cell Signaling Technologies #4060, multiple lots, 1:500 dilution); Vinculin (Sigma Aldrich MAB3574 clone VIIF9, multiple lots, 1:2000 dilution). ..

    Magnetic Resonance Imaging:

    Article Title: Glioblastoma mutations alter EGFR dimer structure to prevent ligand bias.
    Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Human research participants Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Cell Signaling Technology (CST): CST #2231 - Rabbit polyclonal against EGFR pY845 (used at 1:1000 dilution for Western blotting) CST #3777 - Rabbit monoclonal IgG against EGFR pY1068 (used at 1:1000 dilution for Western blotting) 3 nature portfolio | reporting sum m ary M arch 2021 CST #4407 - Rabbit monoclonal IgG against EGFR pY1173 (used at 1:1000 dilution for Western blotting) CST #9106 - Mouse monoclonal IgG1 against pT202/pY204 of ERK1/2 (used at 1:1000 dilution for Western blotting phospho ERK) CST #4696 - Mouse monoclonal IgG1 against (total) ERK1/2 (used at 1:1000 dilution for Western blotting total ERK) CST #3972 - Rabbit polyclonal against Grb2 (used at 1:1000 dilution for Western blotting) CST #7076 - Horse anti-mouse IgG (heavy and light chain) antibody conjugated to horseradish peroxidase (HRP) - used at 1:10,000 dilution as secondary antibody in Western blotting with mouse primaries. .. R&D AF231 - Goat polyclonal IgG against total EGFR (used at 1:1000 dilution for Western blotting total EGFR) R&D HAF017 - Rabbit anti-goat IgG conjugated to horseradish peroxidase (HRP) - used at 1:1000 dilution as secondary antibody in Western blotting with goat primaries.

    Article Title: Developmental mosaicism underlying EGFR-mutant lung cancer presenting with multiple primary tumors.
    Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used EGFR (Cell Signaling Technologies #4267, multiple lots, 1:500 dilution); EGFR pY845 (Cell Signaling Technologies #6963, multiple lots, 1:500 dilution); Akt1/2 (Cell Signaling Technologies #9272, multiple lots, 1:1000 dilution); Akt pS473 (Cell Signaling Technologies #4060, multiple lots, 1:500 dilution); Vinculin (Sigma Aldrich MAB3574 clone VIIF9, multiple lots, 1:2000 dilution). ..

    Article Title: Developmental mosaicism underlying EGFR -mutant lung cancer presenting with multiple primary tumors
    Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used EGFR (Cell Signaling Technologies #4267, multiple lots, 1:500 dilution); EGFR pY845 (Cell Signaling Technologies #6963, multiple lots, 1:500 dilution); Akt1/2 (Cell Signaling Technologies #9272, multiple lots, 1:1000 dilution); Akt pS473 (Cell Signaling Technologies #4060, multiple lots, 1:500 dilution); Vinculin (Sigma Aldrich MAB3574 clone VIIF9, multiple lots, 1:2000 dilution). ..

    Western Blot:

    Article Title: Glioblastoma mutations alter EGFR dimer structure to prevent ligand bias.
    Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Human research participants Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Cell Signaling Technology (CST): CST #2231 - Rabbit polyclonal against EGFR pY845 (used at 1:1000 dilution for Western blotting) CST #3777 - Rabbit monoclonal IgG against EGFR pY1068 (used at 1:1000 dilution for Western blotting) 3 nature portfolio | reporting sum m ary M arch 2021 CST #4407 - Rabbit monoclonal IgG against EGFR pY1173 (used at 1:1000 dilution for Western blotting) CST #9106 - Mouse monoclonal IgG1 against pT202/pY204 of ERK1/2 (used at 1:1000 dilution for Western blotting phospho ERK) CST #4696 - Mouse monoclonal IgG1 against (total) ERK1/2 (used at 1:1000 dilution for Western blotting total ERK) CST #3972 - Rabbit polyclonal against Grb2 (used at 1:1000 dilution for Western blotting) CST #7076 - Horse anti-mouse IgG (heavy and light chain) antibody conjugated to horseradish peroxidase (HRP) - used at 1:10,000 dilution as secondary antibody in Western blotting with mouse primaries. .. R&D AF231 - Goat polyclonal IgG against total EGFR (used at 1:1000 dilution for Western blotting total EGFR) R&D HAF017 - Rabbit anti-goat IgG conjugated to horseradish peroxidase (HRP) - used at 1:1000 dilution as secondary antibody in Western blotting with goat primaries.

    other:

    Article Title: ERBB family fusions are recurrent and actionable oncogenic targets across cancer types
    Article Snippet: AKT pS437 (4058), AKT (2920), ERK pT202/Y204 (9101), ERK (9107), EGFR pY845 (6963) and HER2 pY877 (2241) were purchased from Cell Signaling Technology.



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    Image Search Results


    a , Position of the relevant residues within a partial EGFR protein crystal structure (aligned PDB structures EGFR 696-1022 T790M ( 5gty ) and EGFR 703-985 ( 4zjv )). The dimerization domain is green and the catalytic tyrosine kinase domain blue. b , Schematic of tumor locations in patients 4 and 5. c , Lineage tracing of patient 4 and 5 tumors, derived from WES. Numbers on branches are mutations that accumulated between two nodes, which represent distinct clones identified by WES. Numbers in parentheses are exonic mutations that are not in the FFPE context. d – g , j – m , Functional effect of the H988P- EGFR mutant ( d – g ) or G873E mutant ( j – m ), compared with the WT construct. d , e , j and k are western blots and f , g , l and m are quantifications of the blots immediately above. The pY845 was normalized to vinculin, then total EGFR and finally to the average signal within an experiment for comparison across experiments. phos, phosphorylation. OE, overexpression. EGFR and pY845 are both rabbit antibodies and were run on different blots (processed in parallel), each with their own vinculin loading control, which was used for quantification. A representative vinculin blot is shown in the figure. The pS473 was normalized to a vinculin sample processing control from a matched EGFR blot, then to the average signal within an experiment. AKT and pS473 are both rabbit antibodies and were run on different blots (processed in parallel). Images vertically sliced to juxtapose nonadjacent lanes were run on the same gel ( n = 10 biologically independent samples per figure). Data are presented as mean values ± s.e.m. A two-way ANOVA was performed to determine statistical significance and false recovery rate (FDR) q -values were corrected for multiple comparisons using the Benjamini, Krieger and Yekutieli procedure. h , i , n , Representative images of colony formation by NIH/3T3 cells in soft agar in cells expressing WT or mutant EGFR constructs ( h ). Scale bars, 100 μm. This experiment was repeated 3× with similar results, as quantified in i and n . Quantification data of colonies at least 20 μm in size is presented as mean values ± s.e.m. P values are a one-tailed, unpaired Student’s t -test not corrected for multiple comparisons.

    Journal: Nature Cancer

    Article Title: Developmental mosaicism underlying EGFR -mutant lung cancer presenting with multiple primary tumors

    doi: 10.1038/s43018-024-00840-y

    Figure Lengend Snippet: a , Position of the relevant residues within a partial EGFR protein crystal structure (aligned PDB structures EGFR 696-1022 T790M ( 5gty ) and EGFR 703-985 ( 4zjv )). The dimerization domain is green and the catalytic tyrosine kinase domain blue. b , Schematic of tumor locations in patients 4 and 5. c , Lineage tracing of patient 4 and 5 tumors, derived from WES. Numbers on branches are mutations that accumulated between two nodes, which represent distinct clones identified by WES. Numbers in parentheses are exonic mutations that are not in the FFPE context. d – g , j – m , Functional effect of the H988P- EGFR mutant ( d – g ) or G873E mutant ( j – m ), compared with the WT construct. d , e , j and k are western blots and f , g , l and m are quantifications of the blots immediately above. The pY845 was normalized to vinculin, then total EGFR and finally to the average signal within an experiment for comparison across experiments. phos, phosphorylation. OE, overexpression. EGFR and pY845 are both rabbit antibodies and were run on different blots (processed in parallel), each with their own vinculin loading control, which was used for quantification. A representative vinculin blot is shown in the figure. The pS473 was normalized to a vinculin sample processing control from a matched EGFR blot, then to the average signal within an experiment. AKT and pS473 are both rabbit antibodies and were run on different blots (processed in parallel). Images vertically sliced to juxtapose nonadjacent lanes were run on the same gel ( n = 10 biologically independent samples per figure). Data are presented as mean values ± s.e.m. A two-way ANOVA was performed to determine statistical significance and false recovery rate (FDR) q -values were corrected for multiple comparisons using the Benjamini, Krieger and Yekutieli procedure. h , i , n , Representative images of colony formation by NIH/3T3 cells in soft agar in cells expressing WT or mutant EGFR constructs ( h ). Scale bars, 100 μm. This experiment was repeated 3× with similar results, as quantified in i and n . Quantification data of colonies at least 20 μm in size is presented as mean values ± s.e.m. P values are a one-tailed, unpaired Student’s t -test not corrected for multiple comparisons.

    Article Snippet: The following antibodies were used: EGFR (Cell Signaling Technologies, cat. no. 4267, 1:500 dilution in 5% bovine serum albumin (BSA) in phosphate-buffered saline–Tween (PBST), imaged on LiCor); EGFR pY845 (Cell Signaling Technologies, cat. no. 6963, 1:500 dilution in 5% BSA in PBST, imaged on LiCor); AKT1/2 (Cell Signaling Technologies, cat. no. 9272, 1:1,000 dilution in 5% milk in PBST, imaged on film); AKT pS473 (Cell Signaling Technologies, cat. no. 4060, 1:500 dilution in 5% milk in PBST, imaged on film); and vinculin (Sigma-Aldrich, cat. no. MAB3574, 1:2,000 dilution in 5% BSA in PBST, imaged on LiCor).

    Techniques: Derivative Assay, Clone Assay, Functional Assay, Mutagenesis, Construct, Western Blot, Comparison, Phospho-proteomics, Over Expression, Control, Expressing, One-tailed Test